We are facing C32CS Cannot open port in Micom P543 relay .what is the reason for this?

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It's linked with the Easergy Studio installation. A proper installation of Easergy Studio shall prevent this issue.

After installation ,Please check if ports are set correctly (COM port number) &
Please check, if they used Ethernet connection and propose they use "Quick Connect" with defaults (beside IP addr) if you will communicate though Ethernet